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KMID : 0857020150300020141
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Kosin Medical Journal
2015 Volume.30 No. 2 p.141 ~ p.147
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Analysis of an EGFR mutation by PNA clamping method in lung carcinoid tumors
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김종인:Kim Jong-In
저자없음:No authors listed
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Abstract
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Background: Pulmonary carcinoid tumors consisting of typical carcinoid tumors (TC) and atypical carcinoid tumors (AC) are rare, accounting for 2-5% of all lung tumors. TC is considered a low-grade tumor with a rate of distant metastasis up to 12%. In contrast, ACs are more aggressive tumors, displaying a metastatic rate up to 70%. Surgery is the treatment of choice; however, the current treatment outcomes of metastatic lung carcinoids are discouraging. This study aimed to investigate the EGFR mutation using the PNA-mediated clamping method and to provide basic data for using EGFR-TK1 and its clinical implications.
Materials and Methods: A total of 14 cases that underwent surgery were diagnosed as carcinoid tumors and pathologically classified as TC and AC. The paraffin-embedded tissues were analyzed for EGFR mutations using the PNA-mediated PCR clamping technique. The mutant type was noted in the cases with a △Ct greater than 2.0.
Result: Of 14 cases, eight were AC and six cases were TC. No known EGFR mutation was detected with a △Ct less than 2.0.
Conclusion: The EGFR genotype determined using the PNA-mediated PCR clamping method was wild-type in all pulmonary carcinoid tumors. Therefore, the application of EGFR-TK1 is limited in pulmonary carcinoid tumors.
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KEYWORD
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carcinoid, EGFR mutation, Lung, PNA clamping
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